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New Informatics and Automated Infrastructure to Accelerate New Leads Discovery by High Throughput Screening (HTS)

[ Vol. 16 , Issue. 3 ]

Author(s):

Preston S. Donover, Marlin Yohn, Matthew Sim, Andrew Wright, Sandesh Gowda, Chip Allee, Amanda R. Schabdach and Melvin Reichman   Pages 180 - 188 ( 9 )

Abstract:


The Lankenau Institute for Medical Research Chemical Genomics Center, Inc. has developed a new (patents issued and pending) Nanotube Automated Repository System (NARS) for dynamic storage of millions of ‘single-shot’ samples stored in a new monolithic microtiter-storage tube plate of our own design we call ‘nanotubes.’ We have integrated the NARS with customized software to efficiently access up to 10,000,000 samples stored continuously frozen (–20°C) in a dehumidified enclosure and sealed in a new microtiter NARS plate that is SBS compliant. Additional software was developed to analyze HTS data from orthogonally pooled compound libraries. Following ‘de-convolution’ of pooled HTS data, the software designates confirmatory retest samples to be ‘cherry-picked’ using the NARS. The application of a new, fully-integrated infrastructure for new leads discovery is described in detail. Other applications for our technologies and new infrastructure are discussed.

Keywords:

Automated sample retrieval system (ASRS), bio-repository, drug discovery, HTS, orthogonal pooling, sample management, sample store

Affiliation:

CGC, Inc. (Lankenau Institute for Medical Research Chemical Genomics Center), 100 Lancaster Avenue, Wynnewood, PA 19096, USA.



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