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Development of In Vitro Co-Culture Model in Anti-Cancer Drug Development Cascade

Author(s):

Ruiling Xu and Frances M. Richards   Pages 1 - 7 ( 7 )

Abstract:


Tumour microenvironment is recognized as a major determinant of intrinsic resistance to anticancer therapies. In solid tumour types, such as breast cancer, lung cancer and pancreatic cancer, stromal components provide a fibrotic niche, which promotes stemness, EMT, chemo- and radio-resistance of tumour. However, this microenvironment is not recapitulated in the conventional cell monoculture or xenografts, hence these in vitro and in vivo preclinical models are unlikely to be predictive of clinical response; which might attribute to the poor predictivity of these preclinical drug-screening models. In this review, we summarized recently developed co-culture platforms in various tumour types that incorporate different stromal cell types and/or extracellular matrix (ECM), in the context of investigating potential mechanisms of stroma-mediated chemoresistance and evaluating novel agents and combinations. Some of these platforms will have great utility in the assessment of novel drug combinations and mechanistic understanding of the tumor-stroma interactions.

Keywords:

Tumour microenvironment; drug development; tumour-stroma crosstalk; co-culture

Affiliation:

West China School of Medicine, Sichuan University, Chengdu, 610041,, Cancer Research UK Cambridge Institute, The University of Cambridge, Cambridge CB2 0RE



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