Kulathooran Singaram*, Dhamodaran Marimuthu, Selvakumar Baskaran, Suresh K. Chinaga, Dhivya Shanmugarajan and Thangavel Vadivel Pages 703 - 712 ( 10 )
Aim and Objective: Inspired by the impressive biological properties of pyrrolo[2,3- d]pyrimidine units, the objective of this study was to synthesize some new derivatives of heterocyclic compounds with different substituent’s using solvent-free microwave irradiation conditions from readily available starting material. The synthesized compounds were screened for their in vitro anti-microbial, anti-oxidant, anti-cancer activities and theoretical molecular docking studies.
Material and Methods: Structural elucidation of the synthesized compounds was determined on the basis of various spectroscopic methods. Synthesized compounds have been evaluated for their in vitro antimicrobial activity (MIC) against various microbial strains. After the primary screening, synthesised compounds are further studied for anti-oxidant activity using DPPH assay method, anticancer activity against MCF-7 cell line using MTT assay and molecular docking studies. Moreover, molecular dynamics and simulation was done for best compound using GROMACS.
Results: A series of 2-chloro-7-cyclopentyl-7H-pyrrolo[2,3-d]pyrimidine derivatives 6(a-f), 7(a-c) and 8(a-c) were synthesised using solvent-free microwave irradiation technique. Among all the synthesized compounds, compounds 6e (51.35 μg/mL) and 6f (60.14 µg/mL) showed better activity profile against MCF-7 cell line for breast cancer activity. Compounds 6f and 6d have shown potent antibacterial activity against most of the employed strains, especially against S. pneumoniae, B. cerus and S. aureus. Compound 7a (52.21 µg/mL) showed high potential activity for antioxidant using DPPH assay. Molecular docking study showed good binding of these compounds to the active site of ER- alpha with binding energy ranging from -7.12 kcal/mol to -1.21 kcal/mol. Furthermore, molecular dynamics and simulation was conducted for best pose interacted compound 6e with active site of protein to study its stability and behaviour in nanoscale.
Conclusion: The present research work is intended for facile and efficient green synthesis of various biologically useful potent bio-active molecules from inexpensive and readily available starting substrates under mild reaction condition. These classes of synthesized various heterocyclic compounds holds a great importance to discover newer anti-microbial, anti-oxidant and anti-cancer drugs in future prospects. Further structural modification in these structures will be of interest and may result in compounds having a better therapeutic and biological activity. Hence, this efficient green synthetic protocol and biological results of newly synthesized heterocyclic derivatives are found to be interesting lead molecules for bioactivity in the near future. It could be considered for investigation of their mode of action and for further development.
2-Chloro-7-cyclopentyl-7H-pyrrolo[2, 3-d]pyrimidine, microware irradiation, breast cancer, antioxidant, molecular docking, green synthesis.
Research & Development Centre, Bharathiar University, Coimbatore-641046, Department of Chemistry, Perunthalaivar Kamarajar Institute of Engineering and Technology, (Govt. Puducherry Institution), Karaikal 609603, Department of Chemistry, PG and Research Centre, Sriparamakalyani College, Alwarkurichi 627412, Barrix Agro Sciences Pvt. Ltd., 68A, Peenya, Bengalore- 560058, Department of Biochemistry and Biotechnology, DBT-BIF Facility, Biotechnology Finishing School, Research Centre, Maharani Lakshmi Ammanni College for Women, Bengaluru, Research & Development Centre, Bharathiar University, Coimbatore-641046