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In vitro Cytotoxicity and Genotoxicity Analysis of Ten Tannery Chemicals Using SOS/umu Tests and High-content In vitro Micronucleus Tests

[ Vol. 21 , Issue. 4 ]

Author(s):

Zehao Huang, Na Li, Kaifeng Rao, Cuiting Liu, Zijian Wang* and Mei Ma*   Pages 262 - 270 ( 9 )

Abstract:


Background: More than 2,000 chemicals have been used in the tannery industry. Although some tannery chemicals have been reported to have harmful effects on both human health and the environment, only a few have been subjected to genotoxicity and cytotoxicity evaluations.

Objective: This study focused on cytotoxicity and genotoxicity of ten tannery chemicals widely used in China.

Materials and Methods: DNA-damaging effects were measured using the SOS/umu test with Salmonella typhimurium TA1535/pSK1002. Chromosome-damaging and cytotoxic effects were determined with the high-content in vitro Micronucleus test (MN test) using the human-derived cell lines MGC-803 and A549.

Conclusion: The cytotoxicity of the ten tannery chemicals differed somewhat between the two cell assays, with A549 cells being more sensitive than MGC-803 cells. None of the chemicals induced DNA damage before metabolism, but one was found to have DNA-damaging effects on metabolism. Four of the chemicals, DY64, SB1, DB71 and RR120, were found to have chromosome-damaging effects. A Quantitative Structure-Activity Relationship (QSAR) analysis indicated that one structural feature favouring chemical genotoxicity, Hacceptor-path3-Hacceptor, may contribute to the chromosome-damaging effects of the four MN-test-positive chemicals.

Keywords:

SOS/umu tests, high-content screening, in vitro micronucleus tests, cytotoxicity, genotoxicity, tannery chemicals.

Affiliation:

State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, Chinese Academy of Sciences, Research Center for Eco-Environmental Sciences, Beijing, Chinese Academy of Sciences, Research Center for Eco-Environmental Sciences, Beijing, The Third Affiliated Hospital of Guangzhou Medical University Guangzhou, Guangzhou, State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, University of Chinese Academy of Sciences, College of Resources and Environment, Beijing



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