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Structural Characterization of Gallbladder Stones Using Energy Dispersive X-ray Spectroscopy and X-ray Diffraction

Author(s):

Hassan A. Almarshad, Sayed M. Badawy* and Abdalkarem F. Alsharari   Pages 1 - 6 ( 6 )

Abstract:


Aim and Objective: Formation of the gallbladder stones is a common disease and a major health problem. The present study aimed to identify the structures of the most common types of gallbladder stones using X-ray spectroscopic techniques, which provide information about the process of stone formation. Material and Method: Phase and elemental compositions of pure cholesterol and mixed gallstones removed from gallbladders of patients were studied using energy-dispersive X-ray spectroscopy combined with scanning electron microscopy analysis and X-ray diffraction. Results: The crystal structures of gallstones which coincide with standard patterns were confirmed by X-ray diffraction. Plate-like cholesterol crystals with laminar shaped and thin layered structures were clearly observed for gallstone of pure cholesterol by scanning electron microscopy; it also revealed different morphologies from mixed cholesterol stones. Elemental analysis of pure cholesterol and mixed gallstones using energy-dispersive X-ray spectroscopy confirmed the different formation processes of the different types of gallstones. Conclusion: The method of fast and reliable X-ray spectroscopic techniques has numerous advantages over the traditional chemical analysis and other analytical techniques. The results also revealed that the X-ray spectroscopy technique is a promising technique that can aid in understanding the pathogenesis of gallstone disease.

Keywords:

EDS, XRD, cholesterol, gallstoneEDS, XRD, cholesterol, gallstone

Affiliation:

Faculty of Applied Medical Sciences, Jouf University, Sakaka, National Center for Clinical and Environmental Toxicology, NECTR, Faculty of Medicine, Cairo University, Faculty of Applied Medical Sciences, Jouf University, Sakaka



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