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Expression Levels of A disintegrin-like Metalloproteinase with Thrombospondin motifs-4 and -5 (ADAMTS-4 and ADAMTS-5) in Inflamed and Healthy Gingival Tissues

Author(s):

M. Ayşe Tayman*, İsmail Koyuncu and Nimet Öge Köklü   Pages 1 - 9 ( 9 )

Abstract:


Background: ADAMTS (A disintegrin-like metalloproteinase with thrombospondin motifs) is a group of 19 zinc-dependent metalloproteases known to function in many pathological and physiological processes, such as adhesion, cell fusion, signaling, proteolysis and ECM degradation.

Objectives: The aim of this study was to demonstrate the levels of ADAMTS-4 and -5 in gingival tissues with Stage III-Grade B generalized periodontitis (SIII-GB), Stage III-Grade C generalized periodontitis (SIII-GC) and healthy-control (C) groups.

Methods: The clinical measurements were recorded for each patient. A total of 63 gingival biopsy specimens were obtained from the C (n:20), SIII-GB (n:23) and SIII-GC (n:20) groups. Polymerase chain reaction (Rt-PCR) and immunohistochemical (IHC) examinations were used to determine gene and protein levels.

Results: According to the results of all methods, ADAMTS-4 and -5 expressions were existed in periodontitis and C groups (P> 0.05). Immunostaining for ADAMTS-4 were found to be higher in patients with periodontitis than for ADAMTS-5 (P>0.05). Gene expression levels for ADAMTS-4 and -5 seemed to be up-regulated in subjects diagnosed periodontitis, but the results were not statistically significant (P>0.05). A positive correlation was observed between PPD and ADAMTS-4 mRNA in SIII-GC (p=0.035) and SIII-GB (p=0.015). A positive correlation was determined between ADAMTS-4 mRNA and ADAMTS-5 mRNA in SIII-GC (p=0.037) and SIII-GB (p=0.00).

Conclusion: ADAMTS expression may take part in both pathological and physiological processes in the periodontal tissues, and periodontal destruction may be the result of complex interaction of several pathways with many participants, such as ADAMTS-4 and -5, thus facilitating the exaggeration of periodontal disease.

Keywords:

Matrix metalloproteinase, ADAMTS-4, ADAMTS-5, Periodontal disease, Inflammation

Affiliation:

Department of Periodontology, Faculty of Dentistry, Ankara Yıldırım Beyazıt University, Ankara, Department of Biochemistry, Faculty of Medicine, Harran University, Şanlıurfa, Zekai Tahir Burak, Education and Research Hospital, Department of Pathology, Ankara



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