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Novel Application of Pentabromobenzyl Column for Simultaneous Determination of Eight Antifungal Drugs using High-performance Liquid Chromatography

Author(s):

Maha M. Abou El-Alamin, Maha A. Sultan, Mostafa A. Atia and Hassan Y. Aboul-Enein*   Pages 1 - 12 ( 12 )

Abstract:


Aim: A new, accurate and sensitive reversed-phase high-performance liquid chromatography (RP-HPLC) as an analytical method for the quantitative determination of eight antifungal drugs in spiked human plasma has been described optimized and validated.

Methods and Materials: The analyzed compounds were voriconazole (VOR), luliconazole (LUL), clotrimazole (CLO), tioconazole (TIO), posaconazole (POS), ketoconazole (KET), sertaconazole (SER) and terconazole (TER).

Results: The separation of the analyzed compounds was conducted using a novel pentabromobenzyl column known as COSMOSIL PBB-R (150 mm × 4.6 mm I.D., particle size 5 μm). The analysis of the studied drugs was determined within 14 min using diode array detector and mobile phase consists of: 10 mM potassium dihydrogen phosphate buffer (pH 2.1): Methanol (2: 98 v/v). A linear response was observed for all compounds in the range of concentration studied. Sample preparation was done through liquid-liquid extraction using diethyl ether.

Conclusion: This proposed method was validated in term of linearity, limit of quantification ,limit of detection, accuracy, percision and selectivity. The method was successfully applied for the determination of these drugs in their pharmaceutical formulations and in human plasma samples.

Keywords:

HPLC, Pentabromobenzyl column, Anifungal drugs, Pharmaceutical analysis, Biomedical analysis

Affiliation:

Department of Analytical Chemistry, Faculty of Pharmacy Helwan University, 11795, Cairo, Department of Analytical Chemistry, Faculty of Pharmacy Helwan University, 11795, Cairo, Department of Analytical Chemistry, Faculty of Pharmacy Helwan University, 11795, Cairo, Department of Pharmaceutical and Medicinal Chemistry, Pharmaceutical and Drug Industries Research Division, National Research Centre, Dokki 12622, Giza



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