Submit Manuscript  

Article Details


A Comparative Study on the Incidence, Aggravation, and Remission of Lupus Nephritis Based on iTRAQ Technology

Author(s):

Dongjiang Liao, Xi Ping Cheng*, Nan Li, Kang Li Liang, Hui Fan, Sui Ying Zhang, Xiao Qian Hu, Ping Fan and Yuan Sheng Wu   Pages 1 - 8 ( 8 )

Abstract:


Aim and Objective: Lupus nephritis (LN) is one of the major complications of systemic lupus erythematosus (SLE). The specific mechanisms of pathogenesis, aggravation and remission processes in LN have not been clarified, but is of great need in clinic. Using isobaric tags for relative and absolute quantitation (iTRAQ) technology to screen the functional proteins of LN in mice. Especially under intervention factors of lipopolysaccharide (LPS) and dexamethasone.

Methods: Mrl‐lps mice were intervened with LPS, dexamethasone and normal saline(NS) using intraperitoneal injection, and c57 mice intervened with NS as control. The anti‐ANA antibody enzyme‐linked immunosorbent assay (ELISA) was used to verify disease severity. kidney tissue were collected and processed for iTRAQ to screen out functional proteins closely related to the onset and development of LN. Western blot method and rt‐PCR (real time Polymerase Chain Reaction) were used for verification.

Results: We identified 136 proteins that marked quantitative information. Among them, Hp, Igkv8‐27, Itgb2, Got2 and Pcx proteins showed significant abnormal manifestations.

Conclusion: Using iTRAQ methods, the functional proteins Hp, Igkv8‐27, Itgb2, Got2, and Pcx were screened out for a close relationship with the pathogenesis and development of LN, which is worth further study.

Keywords:

Lupus erythematosus, lupus nephritis, proteomics, iTRAQ Technology, rt‐PCR, pathogenesis.

Affiliation:

Guangzhou Medical University, Guangzhou, Guangdong Province, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, Guangdong Hospital of Traditional Chinese Medicine, Guangzhou, Guangdong Province



Read Full-Text article