Praveen Kumar Posa Krishnamoorthy*, Sekar Subasree, Udhayachandran Arthi, Mobashir Imam, Chirag Gowda and Prasanna DR Pages 1 - 9 ( 9 )
Nipah virus (NiV) is a zoonotic virus of the paramyxovirus family that sporadically breaks out from livestock and spread in human through breathing resulting in indication of encephalitis syndrome. In the current study, T cell epitopes with the NiV W protein antigens were predicted. Modelling of unavailable 3D structure of W protein followed by docking studies of respective Human MHC - class I and MHC - class II alleles predicted was carried out for the highest binding rates. In the computational analysis, epitopes were assessed for immunogenicity, conservation, and toxicity analysis. T – cell based vaccine development against NiV was screened for eight epitopes of Indian - Asian origin. Two epitopes SPVIAEHYY, LVNDGLNII, have been screened and selected for further docking study based on toxicity and conservancy analyses. These epitopes showed a significant score of -1.19 kcal/mol, 0.15 kcal/mol with HLA- B*35:03, HLA- DRB1 * 07:03, allele - Class I and Class II using AutoDock. These two peptides predicted by reverse vaccinology approach are likely to induce immune response mediated by T – cells. Simulation using GROMACS has revealed LVNDGLNII epitope forms more stable complex with HLA molecule and will be useful in developing epitope-based Nipah virus vaccine.
Nipah, vaccination, immunogenicity, conservation, toxicity, docking, simulation
Department Of Biotechnology, Sri Venkateswara College Of Engineering, Pennalur, Sriperumbudur602117, Tamilnadu, Department Of Biotechnology, Sri Venkateswara College Of Engineering, Pennalur, Sriperumbudur- 602117, Tamilnadu, Department Of Biotechnology, Sri Venkateswara College Of Engineering, Pennalur, Sriperumbudur- 602117, Tamilnadu, Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Novels vag 16, 17165 Solna, Stockholm, Department of Biotechnology, Siddaganga Institute of Technology, Tumkuru – 572103, Karnataka, Department of Biotechnology, Siddaganga Institute of Technology, Tumkuru – 572103, Karnataka