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T-cell Epitope-based Vaccine Design for Nipah Virus by Reverse Vaccinology Approach

Author(s):

Praveen Kumar Posa Krishnamoorthy*, Sekar Subasree, Udhayachandran Arthi, Mobashir Imam, Chirag Gowda and Prasanna DR   Pages 1 - 9 ( 9 )

Abstract:


Nipah virus (NiV) is a zoonotic virus of the paramyxovirus family that sporadically breaks out from livestock and spread in human through breathing resulting in indication of encephalitis syndrome. In the current study, T cell epitopes with the NiV W protein antigens were predicted. Modelling of unavailable 3D structure of W protein followed by docking studies of respective Human MHC - class I and MHC - class II alleles predicted was carried out for the highest binding rates. In the computational analysis, epitopes were assessed for immunogenicity, conservation, and toxicity analysis. T – cell based vaccine development against NiV was screened for eight epitopes of Indian - Asian origin. Two epitopes SPVIAEHYY, LVNDGLNII, have been screened and selected for further docking study based on toxicity and conservancy analyses. These epitopes showed a significant score of -1.19 kcal/mol, 0.15 kcal/mol with HLA- B*35:03, HLA- DRB1 * 07:03, allele - Class I and Class II using AutoDock. These two peptides predicted by reverse vaccinology approach are likely to induce immune response mediated by T – cells. Simulation using GROMACS has revealed LVNDGLNII epitope forms more stable complex with HLA molecule and will be useful in developing epitope-based Nipah virus vaccine.

Keywords:

Nipah, vaccination, immunogenicity, conservation, toxicity, docking, simulation

Affiliation:

Department Of Biotechnology, Sri Venkateswara College Of Engineering, Pennalur, Sriperumbudur602117, Tamilnadu, Department Of Biotechnology, Sri Venkateswara College Of Engineering, Pennalur, Sriperumbudur- 602117, Tamilnadu, Department Of Biotechnology, Sri Venkateswara College Of Engineering, Pennalur, Sriperumbudur- 602117, Tamilnadu, Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institute, Novels vag 16, 17165 Solna, Stockholm, Department of Biotechnology, Siddaganga Institute of Technology, Tumkuru – 572103, Karnataka, Department of Biotechnology, Siddaganga Institute of Technology, Tumkuru – 572103, Karnataka



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