Submit Manuscript  

Article Details


GC-MS Based Metabolic Profiling of Parkinson’s Disease with Glutathione S-transferase M1 and T1 Polymorphism in Tunisian Patients

Author(s):

Amal Rebai*, Tuba Reçber, Emirhan Nemutlu, Chahra Chbili, Sevinç Kurbanoglu, Sedef Kir, Sana Ben Amor, Sibel A Özkan and Saad Saguem   Pages 1 - 8 ( 8 )

Abstract:


Aim and Objective: Parkinson’s disease (PD) is the second neurodegenerative disease. It is a multifactorial disorder (aging, environmental and genetic factors). Metabolomics can help to explore the biomarker profiles for aging. Recent studies showed an association between the glutathione S-transferases (GSTs) polymorphisms and PD risk. The purpose of this study was to evaluate the association of this genetic polymorphism and the metabolomic profile in PD Tunisian patients, in order to identify effective biomarkers in the genetic differentiation.

Materials and Methods: In this study, the metabolomic profile changes related to GSTs polymorphism were searched in 54 Tunisian PD patients treated with L-dopa, using a gas chromatography-mass spectrometry (GCMS) technique.

Results: The study results showed that mannose, methyl stearate, and three unknown metabolites, increased in GSTM1 positive patients; and glycolic acid, porphine, monomethyl phosphate, fumaric acid, and three others unknown metabolites, decreased in GSTM1 positive patients. Subsequently, the levels of, glycolic acid, erythronic acid, lactic acid, citric acid, fructose, stearic acid, 2-amino-2-methyl-1,3-propanediol and three unknown metabolites increased in GSTT1 positive patients; and the levels of proline, valine and two unknown metabolites decreased in GSTT1 positive patients.

Conclusion: All these altered metabolites are related to energy metabolism and we may conclude that GSTs polymorphism based shifting in the energy metabolism and leading to oxidative stress.

Keywords:

Glutathione S-transferases polymorphism, Metabolomic Profile, Metabolites, Oxidative stress, Parkinson’s disease, Tunisian patient.

Affiliation:

Metabolic Biophysics Laboratory, Department of Biophysics, Faculty of Medicine Sousse, Sousse University, Sousse, Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Metabolic Biophysics Laboratory, Department of Biophysics, Faculty of Medicine Sousse, Sousse University, Sousse, Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, Ankara, Neurology Department of “Sahloul” University Hospital Center (UHC) - Sousse University, Sousse, Department of Analytical Chemistry, Faculty of Pharmacy, Ankara University, Ankara, Metabolic Biophysics Laboratory, Department of Biophysics, Faculty of Medicine Sousse, Sousse University, Sousse



Read Full-Text article