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Electroanalytical Analysis of Guaifenesin on Poly(Acridine Orange) Modified Glassy Carbon Electrode and its Determination in Pharmaceuticals and Serum Samples

Author(s):

Hasan Işik, Gökçe Öztürk, Fatma Ağin* and Dilek Kul   Pages 1 - 10 ( 10 )

Abstract:


Background: Electroanalytical methods are very functional to detect drugs in pharmaceuticals (tablets, syrups, suppositories, creams and ointments) and biological samples.

Objective: In this study, it is aimed to make selective, sensitive, simple, fast and low cost electrochemical analysis of expectorant drug guaifenesin in pharmaceuticals and serum samples.

Method: Differential pulse adsorptive stripping voltammetric method for determination of guaifenesin on a poly(acridine orange) modified glassy carbon electrode has been developed. Glassy carbon electrode was modified with electropolymerization of acridine orange monomer for sensitive determination of guaifenesin. Guaifenesin provided highly reproducible and well-defined irreversible oxidation peaks at +1.125 V and +1.128 V (vs. Ag/AgCl) in the selected supporting electrolyte and human serum samples, respectively.

Results: Under optimized conditions, linear response of peak current on the concentration of guaifenesin has been obtained in the ranges of 2.00×10-7 to 1.00×10-4 M in Britton Robinson buffer solution at pH 7.0 and 4.00×10-7 to 1.00×10-4 M in serum samples. The precision of the method was detected by intraday and inter-day repeatability studies in the supporting electrolyte and serum samples media.

Conclusion: The analytical applicability of the proposed method exhibited satisfying determination results for guaifenesin from pharmaceutical dosage forms (syrup) and human serum samples without any pre-separation procedure.

Keywords:

Differential pulse adsorptive stripping voltammetry, electro-oxidation, guaifenesin, expectorant, poly(acridine orange) modified glassy carbon electrode, voltammetry

Affiliation:

Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Teknik University, Trabzon, Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Teknik University, Trabzon, Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Teknik University, Trabzon, Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Teknik University, Trabzon



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