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Simple and Fast Determination of Terbinafine in Human Urine by Dilute and Shoot HPLC-DAD Using a Core-Shell Column

Author(s):

Sercan Yıldırım*, Gökhan Demirdaş, Mert Fidan and Ahmet Yaşar   Pages 1 - 10 ( 10 )

Abstract:


Background: Terbinafine is an allylamine antifungal which is effective against many fungi, dermatophytes and moulds. Analytical methods are required for the determination of terbinafine in biological fluids to perform therapeutic drug monitoring and pharmacokinetic studies.

Objective: The aim of this study was to develop and validate a novel and fast method combining dilute and shoot approach and high-performance liquid chromatography coupled with photodiode array detection for the determination of terbinafine in human urine.

Methods: Chromatographic parameters including mobile phase composition, pH, flow rate and injection volume was assessed and optimized. The separation of terbinafine and naproxen (internal standard) was achieved within 3 min using a C18 core-shell column (Raptor ARC-18, 100 x 4.6 mm, 2.7 µm) under isocratic conditions. Samples were eluted from the column at the flow rate of 1.4 mL/min using a mobile phase containing 0.2% triethylamine in water (pH 3.4 with formic acid): acetonitrile (45:55, v/v).

Results: Presented technique was linear in the range of 25-2000 ng/mL. Intra- and inter-day reproducibility at four quality control levels (25, 200, 750 and 1500 ng/mL) was less than 7%, with relative errors ranging from -5.40% to 5.91%. Limit of detection was 12.60 ng/mL. Developed method has three main advantages compared to existing methods: simplicity and greenness of sample preparation, use of core-shell column and short analysis time.

Conclusion: The results of this study indicate that the combination of dilute and shoot approach and core-shell column can be regarded as an advantageous application for the fast determination of terbinafine in urine.

Keywords:

Core-shell columns, Dilute and shoot approach, Fast analysis, High performance liquid chromatography, Terbinafine, Urine.

Affiliation:

Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, Department of Analytical Chemistry, Faculty of Pharmacy, Karadeniz Technical University, Trabzon



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