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A potential MRI agent and an anticancer drug encapsulated within CPMV virus-like particles

Author(s):

Alaa A A Aljabali*, Lorca Alzoubi, Yassmen Hamzat, Alaa Alqudah, Mohammad A. Obeid, Mazhar S. Al Zoubi, Raed M Ennab, Walhan Alshaer, Khaled Albatayneh, Bahaa Al‑Trad, Dana A. Alqudah, Dinesh Kumar Chellappan, Gaurav Gupta, Murtaza M. Tambuwala, Dua Kamal and David J Evans   Pages 1 - 15 ( 15 )

Abstract:


Background: Virus nanoparticles have been extensively studied over the past decades for theranostics applications. Viruses are well-characterized, naturally occurring nanoparticles that can be produced in high quantity with a high degree of similarity in both structure and composition.

Objectives: The plant virus cowpea mosaic virus (CPMV) has been innovatively used as a nanoscaffold. Utilization of the internal cavity of empty virus-like particles (VLPs) for the inclusion of therapeutics within the capsid has opened many opportunities in drug delivery and imaging applications.

Methods: The encapsidation of magnetic materials and anticancer drugs was achieved. SuperscriptCPMV denotes molecules attached to the external surface of CPMV and CPMVSubscript denotes molecules within the interior of the capsid.

Results: Here, the generation of novel VLPs incorporating iron-platinum nanoparticles TCPMVFePt and cisplatin (Cis) (TCPMVCis) is reported. TCPMVCis have a cytotoxic IC50 of TCPMVCis on both A549 and MDA-MB-231 cell lines of 1.8 μM and 3.9 μM respectively after 72 hours of incubation. The TCPMVFePt were prepared as potential MRI contrast agents.

Conclusions: Cisplatin loaded VLP (TCPMVCis) is shown to enhance cisplatin cytotoxicity in cancer cell lines, and its potency increased 2.3-folds.

Keywords:

Virus-like particles, Viruses, Bioconjugation, Drug Delivery, Cisplatin, Nanomedicine

Affiliation:

Faculty of Pharmacy, Department of Pharmaceutics and Pharmaceutical Technology, Yarmouk University- Irbid, Faculty of Pharmacy, Department of Medicinal Chemistry & Pharmacognosy, Yarmouk University- Irbid, Faculty of Pharmacy, Department of Pharmaceutics and Pharmaceutical Technology, Yarmouk University- Irbid, Faculty of Pharmacy, Department of Pharmaceutics and Pharmaceutical Technology, Yarmouk University- Irbid, Faculty of Pharmacy, Department of Pharmaceutics and Pharmaceutical Technology, Yarmouk University- Irbid, Faculty of Medicine, Department of Basic Medical Studies-Yarmouk University- Irbid, Faculy of Medicine, Department of Clinical Sciences, Yarmouk University- Irbid, Cell Therapy Center, The University of Jordan, Amman 11942, Department of Biological Sciences, Yarmouk University—Faculty of Science, Irbid 566, Department of Biological Sciences, Yarmouk University—Faculty of Science, Irbid 566, Cell Therapy Center, The University of Jordan, Amman 11942, Department of Life Sciences, School of Pharmacy, International Medical University, Bukit Jalil, Kuala Lumpur 57000, School of Pharmacy, Suresh Gyan Vihar University, Jagatpura, Jaipur 302017, SAAD Centre for Pharmacy and Diabetes, School of Pharmacy and Pharmaceutical Science Ulster University, Coleraine, Discipline of Pharmacy, Graduate School of Health, University of Technology, Sydney, NSW 2007, John Innes Centre, Norwich Research Park, Norwich, NR4 7UH



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