Submit Manuscript  

Article Details


Acute Inflammation and Oxidative Stress Induced by Lipopolysaccharide and the Ameliorative Effect of Stingless Bee Honey

Author(s):

Yazan Ranneh*, Ayman M. Mahmoud*, Abdulmannan Fadel, Mohammed Albujja, Abdah Md Akim, Hasiah Ab. Hamid and Huzwah Khazaai   Pages 1 - 13 ( 13 )

Abstract:


Background: Systemic acute inflammation is the hallmark of sepsis and associated with multiple organ dysfunction.

Objective: This study investigated the potential of stingless bee honey (SBH) to suppress lipopolysaccharide (LPS)-induced systemic acute inflammation in rats and to reveal the probable mechanism of action.

Methods: Rats received 4.6 and 9.2 g/kg SBH for 7 days followed by a single injection of LPS and samples were collected after 6 h.

Results: LPS induced liver, kidney, heart and lung injury manifested by increased serum transaminases, alkaline phosphatase, creatine kinase, creatinine and urea, along with multiple histological alterations, particularly, leukocyte infiltrations. Pro-inflammatory cytokines were elevated in serum, and NF-κB p65, p38 MAPK and HMGB-1 were significantly increased in different tissues of LPS-challenged rats. SBH prevented tissue injury, ameliorated pro-inflammatory cytokines, and suppressed NF-κB p65, p38 MAPK and HMGB-1 in rats received LPS. In addition, SBH diminished reactive oxygen species (ROS) production, lipid peroxidation and oxidative DNA damage, and enhanced glutathione and Nrf2 in LPS-induced rats

Conclusion: SBH prevents systemic acute inflammation by suppressing NF-κB, p38 MAPK, HMGB-1, oxidative stress and tissue injury in rats. Thus, SBH may represent an effective anti-inflammatory nutraceutical, pending further mechanistic studies.

Keywords:

Inflammation, Sepsis, ROS, HMGB-1, NF-κB, Nrf2, MAPK

Affiliation:

Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Physiology Division, Zoology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62514, Sport and Exercises Sciences School, Faculty of Science, Liverpool John Moores University, Liverpool L3 5UG, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor



Read Full-Text article