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Exploring the Mechanism of Swertia mussotii Franch. for Hepatoprotective Effects with iTRAQ LC-MS/MS

Author(s):

Haixia Yun, Xinyu Wu, Yiwei Ding, Wendou Xiong, Xianglan Duan, Hui Gao, Yingzhong Yang and Zhi Chen*   Pages 1 - 8 ( 8 )

Abstract:


Background and Objective: A Tibetan traditional herb named Swertia mussotii Franch., also called “Zangyinchen” by the local people of Qinghai-Tibet area, has been used to protect the liver from injury for many years. However, the curative effect and molecular mechanism of the herb have not been demonstrated clearly.

Materials and Methods: In our study, serum alanine aminotransferase, aspartate aminotransferase, total bilirubin levels were examined after S. mussotii Franch. treatment in the acute liver injury of the carbon tetrachloride-induced rat model. Then, Proteome Analysis was applied to explore the potential mechanism of SMT for hepatoprotective effects after iTRAQLC-MS/MS analysis (isobaric tag for relative and absolute quantification-liquid chromatograph-mass spectrometer with tandem mass spectrometry).

Results: Serum results showed, alanine aminotransferase, aspartate aminotransferase, total bilirubin levels of rats with acute liver injury were all improved with SMT treatment. Moreover, Proteome Analysis suggested that, with S. Mussotii Franch. treatment, the levels of lipid catabolic process and lipid homeostasis were all enhanced. And the results of protein-protein interaction (PPI) analysis illustrated that these proteins assembled in PPI networks were found almost significantly enriched in response to lipid, negative regulation of lipase activity, response to lipopolysaccharide etc. Furthermore, the downregulated MRP14 and MRP8 proteins were found involved in the lipid metabolism, which may indicate the mechanism of SMT protection liver from ALI induced by carbon tetrachloride.

Conclusion: SMT herb could play a role in hepatoprotection and alleviate the effect of acute liver injury by impacting the lipid metabolism associated biological process.

Keywords:

Acute liver injury, Swertia mussotii Franch., proteomics, lipid metabolism, hepatoprotective, differential proteins.

Affiliation:

Medical College of Qinghai University, Xining 810001, Qinghai, Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibetan Plateau, School of Life Science, Qinghai Normal University, Xining 810008, Qinghai, Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibetan Plateau, School of Life Science, Qinghai Normal University, Xining 810008, Qinghai, Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibetan Plateau, School of Life Science, Qinghai Normal University, Xining 810008, Qinghai, Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibetan Plateau, School of Life Science, Qinghai Normal University, Xining 810008, Qinghai, Medical College of Qinghai University, Xining 810001, Qinghai, Medical College of Qinghai University, Xining 810001, Qinghai, Key Laboratory of Medicinal Animal and Plant Resources of the Qinghai-Tibetan Plateau, School of Life Science, Qinghai Normal University, Xining 810008, Qinghai



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