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Biomarker Analysis Based Chemoprofiling of Polyherbal Ayurvedic Formulation Containing Vitis vinifera L. by Validated UPLC-MS/MS Method

Author(s):

Prerna Goel, Vidhu Aeri*, Rikeshwer Prasad Dewangan and Rehan Abdur Rub   Pages 1 - 14 ( 14 )

Abstract:


Background: Drakshasava is one of the commercial Ayurvedic medicine from India, prepared from grapes and spices. It is believed to address health imbalances and claimed to be beneficial for weakness bleeding disorders and various inflammatory diseases. It reported pharmacological activities such as diuretic, cardioprotective and antimicrobial. Being a polyherbal mixture, it faces challenges in its standardization and quality control.

Objective The aim of the present study is to develop a validated UPLC-MS/MS method for simultaneous quantification of ten polyphenolic biomarkers in Drakshasava. It explores the effect of Vitis vinifera L. and additional herbs on fermentation with respect to bioactive compounds through successive addition method.

Method: The MS methods were optimized in multiple-reaction monitoring (MRM) mode with ESI while chromatographic separation was achieved on an Acquity UPLC BEH C18 column using both isocratic and gradient elution in water and acetonitrile containing 0.1% formic acid.

Results: The developed method was validated as per ICH-Q2B guidelines and found to be within the assay variability limits. Gallic acid was found to be the most abundant marker in all the samples followed by resveratrol. The content of all the markers has found to be increased significantly post fermentation, compared to decoction except kaempferol. The successive addition of prashpeka drvya (minor herbs) in the formulation, show variability at different stages, with respect to the selected markers and does not infer major changes in the chemical profiling of final product.

Conclusion: The developed method was found rapid, accurate, reliable and highly sensitive for the simultaneous quantification of selected biomarkers in Drakshasava. The research is the first chemometric report in standardization of Drakshasava by validated UPLC-MS/MS method. It may prove to be useful tool for development of new phyto-pharmaceutical drug and further quality control of other poly herbal formulations.

Keywords:

Validation, Biomarkers, UPLC-MS/MS, Polyherbal, Chemoprofiling, Vitis vinifera L., Ayurvedic formulation, Drakshasava

Affiliation:

Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard (Deemed to be University), New Delhi-110062, Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard (Deemed to be University), New Delhi-110062, Department of Pharmaceutical Chemistry, School of Pharmaceutical Education and Research, Jamia Hamdard (Deemed to be University), New Delhi-110062, Nanomedicine Research Laboratory, School of Pharmaceutical Education and Research, Jamia Hamdard (Deemed to be University), New Delhi 110062



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