Submit Manuscript  

Article Details


Identification of a MiRNA-mRNA Regulatory Network in Colorectal Cancer

Author(s):

Ming-fu Cui, Yuan-yu Wu, Ming-yan Chen, Yang Zhao, Song-yun Han, Rui-jie Wang, Haiyan Zhang and Xue-dong Fang*   Pages 1 - 11 ( 11 )

Abstract:


Background: Colorectal cancer (CRC) is the fourth most prevalent cancer in the world. However, the molecular mechanism underlying CRC is largely unknown.

Objective: To explore the pathogenic mechanism of CRC and to facilitate better diagnosis and treatment of this disease.

Methods: Differentially expressed miRNAs (DEMs) and genes (DEGs) in CRC vs. Control samples from the miRNA expression data in GSE115513 and the miRNA and mRNA expression data in TCGA-COAD dataset were screened, followed by the construction of miRNA-mRNA regulatory network. Functional and pathway enrichment analysis, protein-protein interaction (PPI) analysis, and survival analysis were then performed for these DEGs and DEMs.

Results: We identified 64 DEMs from GSE115513 dataset and 265 DEMs and 2218 DEGs from TCGA-COAD dataset. miR-27a-3p was a hub DEM with the highest degree in miRNA-mRNA network, while GRIN2B and PCDH10 were hub DEGs targeted by multiple miRNAs, including miR-27a-3p. SNAP25 and GRIN2B were also hub DEGs with the highest degree of interactions in PPI network. These DEMs and DEGs were significantly enriched in multiple KEGG pathways, including proteoglycans expression and cAMP signaling pathway in cancer. Finally, seven DEGs, including FJX1 Dsc2, and hsa-miR-375, were revealed to be correlated with CRC prognosis.

Conclusions: Aberrant expression of genes and miRNAs were involved in the pathogenesis of CRC probably by regulating proteoglycans expression and cAMP signaling. miR-27a-3p, PCDH10, GRIN2B, FJX1, Dsc2, and hsa-miR-375 were identified as potential targets for understanding the pathogenic mechanism of CRC. In addition, FJX1, Dsc2 and hsa-miR-375 were identified as potential predictive markers for CRC prognosis.

Keywords:

Colorectal cancer, TCGA-COAD, miRNA-mRNA regulatory network, microRNA, survival analysis, biomarker.

Affiliation:

Department of Gastrointestinal and Colorectal Surgery, The Third Hospital of Jilin University, Changchun, Jilin 130033, Department of Gastrointestinal and Colorectal Surgery, China-Japan Union Hospital, Jilin University, Changchun, Jilin 130033, Department of General Surgery, General Hospital of FAW (The Fourth Hospital of Jilin University), Changchun, Jilin 130033, Department of Anorectal Surgery, Siping Central People’s Hospital, Jilin University, Siping 136000, Jilin, Department of Emergency, Tonghua Central Hospital, Jilin University, Tonghua 134000, Jilin, Department of Gastrointestinal Surgery, Shengli Oilfield Central Hospital Affiliated to Binzhou Medical College, Dongying 257000, Shandong, Department of Gastrointestinal and Colorectal Surgery, The Third Hospital of Jilin University, Changchun, Jilin 130033, Department of Gastrointestinal and Colorectal Surgery, The Third Hospital of Jilin University, Changchun, Jilin 130033



Read Full-Text article