Noureddine Djebli, Mohammad Rais Mustafa, Merve Keskin* and Sevgi Kolayli Pages 1 - 9 ( 9 )
Aim and Objective: This study aimed at investigating the gastro-protective effects of Algerian Sahara (Sidr) honey from Apis mellifera intermissa against HCl/Ethanol-induced gastric ulcers in rats.
Material and Methods: Total phenolic content, antioxidant activity and phenolic compounds were determined. Than, Three groups of rats (control, HCl/ Ethanol induced ulcer, and orally administered honey) were used for the determination of gastro protective effect of Sidr honey.
Results: Total phenolic content, total flavonoid content, and DPPH activity of honey sample was determined as 47.35±3.35 mg GAE/ 100 g, 2.13±0.17 mg QE/ 100 g, and 229.24±0.02 mg/mL, respectively. Oral pretreatment of rats with honey (1.2 g/Kg body weight orally at interval of 2 days) protected gastric mucosa against HCl/Ethanol induced damage by decreasing ulcer score, the volume and acidity of gastric juice and increasing pH.
Conclusion: These results were confirmed by the histological assessment which demonstrated a significant gastro protective activity of Saharian (Sidr) honey against HCl/Ethanol-induced stomach ulcer. Plasma tumor necrosis factor-!, IL-6 and PGE2were also measured. Sahara honeys significantly decreased the plasma TNF-!, PGE2, and IL-6 concentrations.
Gastric ulcer, Sahara honey, HCl/Ethanol, ulcer score, TNF, IL-6, PGE2
Pharmacognosy & Api-Phytotherapy Laboratory, Mostaganem University, Faculty of Medicine University of Malaysia, 50603 Kuala Lumpur, Vocational School of Health Service, Bilecik Seyh Edebali University, 11230, Bilecik, Department of Chemistry, Faculty of Sciences, Karadeniz Technical University, 61080 Trabzon