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Neobavaisoflavone Demonstrates Valid Anti-tumor Effects in Non-Small- Cell Lung Cancer by Inhibiting STAT3

Author(s):

Xueding Cai, Feng Zhou, Xiaona Xie, Dandan Zheng, Yulei Yao, Chengguang Zhao, Xiaoying Huang and Ke Hu*   Pages 1 - 9 ( 9 )

Abstract:


Aim and Objective: Lung cancer is the most common cancer which contributes to the majority of death caused by cancer where non-small-cell lung cancer (NSCLC) accounts for approximately 85%. To treat NSCLC, STAT3 has been identified as a target with therapeutic potential. The neobavaisoflavone (NBIF) is one of the flavonoids of traditional Chinese medicine Psoralea corylifolial.

Materials and Methods: Human NSCLC cell lines, PC-9, H460 and A549, were applied to determine NBIF’s antiproliferative effects through cell viability and colony formation detection. The effect of NBIF on cell apoptosis was determined through Flow cytometry-based assay. Western blotting was used in this study to confirm the levels of P-STAT3 and Bcl-2 and Bax which are apoptotic proteins.

Results: It was observed that NBIF could decrease the cell viability and migration and induce apoptosis in human NSCLC cell lines dose-dependently. Levels of P-STAT3, as well as the downstream signals of STAT3 pathway, were downregulated, suggesting that the tumor-suppression effects of NBIF might be related to the inhibition of STAT3 signaling. Furthermore, NBIF could contribute to the upregulation of BAX and downregulation of BCL2.

Conclusion: NBIF might perform the anti-NSCLC efficacy as a result of the inhibition on STAT3 pathway. Besides, our work suggests that NBIF could provide therapeutic alternatives for NSCLC.

Keywords:

Neobavaisoflavone, NSCLC, STAT3, apoptosis, antitumor

Affiliation:

Department of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan University, Zhangzhidong Road No.99, Wuhan, Hubei 430060, Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, Chemical Biology Research Center, School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, Division of Pulmonary and Critical Care Medicine, The First Affiliated Hospital of Wenzhou Medical University, Key Laboratory of Heart and Lung, Wenzhou, Zhejiang 325000, Department of Respiratory and Critical Care Medicine, Renmin Hospital of Wuhan University, Zhangzhidong Road No.99, Wuhan, Hubei 430060



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