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MicroRNA-96 is Downregulated in Sepsis Neonates and Attenuates LPSInduced Inflammatory Response by Inhibiting IL-16 in Monocytes

Author(s):

Chunlei Zhang, Xiuting Li, Na Liu, Zijian Feng and Chengyuan Zhang*   Pages 1 - 7 ( 7 )

Abstract:


Background: Neonatal sepsis (NS) remains one of the leading causes of mortality among newborns. This study found the deregulated microRNA-96 (miR-96) in NS neonates, and aimed to evaluate the clinical significance of miR-96, as well as its effect on LPS-induced inflammatory response in monocytes. In addition, the relationship of interleukin-16 (IL16) and miR-96 was investigated to understand the underlying mechanisms.

Methods: Expression of miR-96 was examined using real-time quantitative PCR. Monocytes stimulated by LPS was used to mimic excessive inflammation in the pathogenesis of NS. The enzyme-linked immunosorbent assay was applied to evaluate pro-inflammatory cytokines levels. A luciferase reporter assay was used to confirm the interaction between miR-96 and IL16.

Results: Serum miR-96 expression was decreased in NS newborns and had considerable diagnostic value for NS screening. LPS inhibited miR-96 expression in monocytes, and the overexpression of miR-96 could reverse the effects of LPS on the inflammation of monocytes. IL-16 was a target gene of miR-96 and negatively correlated with miR-96 levels in NS neonates. The inhibited inflammatory responses induced by miR-96 overexpression was abolished by the elevated IL-16 in monocytes.

Conclusion: All the data reveal that serum decreased miR-96 may serve as a candidate non-invasive biomarker for NS diagnosis. In addition, miR-96 inhibits LPS-induced inflammatory responses by targeting IL-16 in monocytes. The miR96/IL-16 axis may provide novel therapeutic targets for NS treatment.

Keywords:

MicroRNA-96, Neonatal sepsis, Diagnosis, Inflammation, Monocyte, Interleukin-16

Affiliation:

Neonatology Department, Weifang Maternal and Child Health Hospital, Weifang 261011, Children's Rehabilitation Department, Weifang Maternal and Child Health Hospital, Weifang 261011, Neonatology Department, Weifang Maternal and Child Health Hospital, Weifang 261011, Neonatology Department, Weifang Maternal and Child Health Hospital, Weifang 261011, Neonatology Department, Weifang Maternal and Child Health Hospital, Weifang 261011



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