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Phyto-chlorophyllin Prevents Food Additive Induced Genotoxicity and Mitochondrial Dysfunction via Cytochrome c Mediated Pathway in Mice Model

Author(s):

Asmita Samadder*, Sudatta Dey, Priyanka Sow, Ruchira Das, Sisir Nandi, Jayeeta Das*, Banani Bhattacharjee, Arnob Chakrovorty, Maharaj Biswas and Pradeepta Guptaroy   Pages 1 - 10 ( 10 )

Abstract:


Objectives: The issue of food-additive-toxicity causing several health hazards needs to be therapeutically managed in an immediate effect. Alloxan, a food additive, used for whitening and shining of flour, is capable of inducing genotoxicity, diabetes, and associated mitochondrial dysfunction. Therefore, to explore a non-toxic, phyto-based compound which could delay the onset of diabetes and prevent the multitude of damage associated therein, Chlorophyllin (CHL), was selected for our study having been reported to exhibit anti-cancer, anti-diabetes, anti-inflammatory responses. Therefore, the objectives of the present study aimed to evaluate the protective role of CHL in controlling genotoxicity, glucose imbalance, and associated cytochrome c mediated mitochondrial signaling dysfunction against food-additive-induced genotoxicity, diabetic state, and its complexities in mice model in vivo.

Method: Mice were pre-treated with CHL through oral gavage before they were exposed to alloxan. Diabetic markers, antioxidant enzyme profile, chromosomal study, mitochondrial functioning factors, and expression of proteins were checked against food-additive injected mice.

Results: The results revealed that CHL pre-treatment could delay the onset of diabetes, restrict alloxan-induced elevation of blood glucose, reduce DNA-damage and chromosomal aberration, optimize enzymatic profile (glucokinase, pyruvate, insulin), and modulates protein expression (insulin, IRS1, IRS2, GLUT2). Further, CHL-pre-treatment could stabilize mitochondrial-membrane-potential, intracellular calcium ion, ATP/ADP ratio, ATPase activity thereby maintaining optimum functioning of cytochrome-c, bcl2, and caspase3 mitochondrial protein.

Conclusion: Therefore, the present study reports for the first time, the screening of phyto-based bioactive CHL for preventing/limiting the extent of food-additive-induced genotoxicity and mitochondrial dysfunction and serve as an advanced therapeutic tool in the management of diabetes.

Keywords:

Food additive, Chlorophyllin, diabetes, mitochondria, cytochrome c, mice

Affiliation:

Cytogenetics and Molecular biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia-741235, Cytogenetics and Molecular biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia-741235, Cytogenetics and Molecular biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia-741235, Cytogenetics and Molecular biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia-741235, Department of Pharmaceutical Chemistry, Global Institute of Pharmaceutical Education and Research (Affiliated to Uttarakhand Technical University). Kashipur-244713, Cytogenetics and Molecular biology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia-741235, Department of Zoology, Dum Dum Motijheel College, 1, Motijheel Avenue, Kolkata 700074, Department of Zoology, Dum Dum Motijheel College, 1, Motijheel Avenue, Kolkata 700074, Endocrinology Laboratory, Department of Zoology, University of Kalyani, Kalyani, Nadia-741235, Dum Dum Motijheel College, 1, Motijheel Avenue, Kolkata 700074



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