Aiswarya Anilkumar Ajitha, Sri SivaKumar, Gayathri Viswanathan, Sabulal Baby and Prabath Gopalakrishnan Biju*
Background: Over the last few decades, there has been a stupendous change in the area of drug delivery using particulate delivery systems, with increasing focus on nanoparticles in recent times. Nanoparticles helps to improve and alter the pharmacodynamic properties and pharmacokinetics of various types of drug molecules. These features help to protect the drug entity in the systemic circulation, access of the drug to the chosen sites, and to deliver the drug in a controlled and sustained rate at the site of action.
Objective: Nanoparticle based targeted delivery of anti-inflammatory drugs/signal modulatory agents to the cytoplasm or nuclei of the targeted cell can significantly enhance the precision and efficacy of intended therapeutic activity. To this end, we report ligand free, enhanced intra-nuclear delivery model of anti-inflammatory therapeutics via PDMS nanoparticles.
Method: PDMS nanoparticles were prepared by sacrificial silica template-based approach and details of their characterization for suitability as a nanoparticle-based delivery material is detailed herein.
Results: Biological evaluation for compatibility was carried out and the results showed that the PDMS nanoparticle has no toxicity on RAW 264.7 cells in the concentration range of 10, 20, 40, 60, 80, 100 and 120 μg/mL in culture. Biocompatibility and absence of toxicity was determined by morphological examination and cell viability assays. Drug loading and release kinetics were carried out with the anti-inflammatory drug Diclofenac.
Conclusion: In this paper we clearly demonstrate the various aspects of nanoparticle articulation, characterization, effect of their characteristics and their applications as a non-toxic drug delivery molecule for its potential applications in therapeutic delivery of drugs for sustained release.
Nanoparticle, PDMS, Anti-inflammatory, Drug delivery, Toxicity, Biocompatibility
Department of Biochemistry, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581, Department of Chemical Engineering, Indian Institute of Technology, Kanpur, Uttar Pradesh208016, Phytochemistry and Phytopharmacology Division, KSCSTE-Jawaharlal Nehru Tropical Botanic Garden and Research Institute (KSCSTE-JNTBGRI), Palode, Thiruvananthapuram, Kerala 695562, Phytochemistry and Phytopharmacology Division, KSCSTE-Jawaharlal Nehru Tropical Botanic Garden and Research Institute (KSCSTE-JNTBGRI), Palode, Thiruvananthapuram, Kerala 695562, Department of Biochemistry, University of Kerala, Kariavattom campus, Thiruvananthapuram, Kerala 695581