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Identification of a Novel Epithelial-to-Mesenchymal-related Gene Signature in Predicting Survival of Patients with Hepatocellular Carcinoma

Author(s):

Simeng Xiao, Junjie Hu, Na Hu, Lei Sheng, Hui Rao and Guohua Zheng*  

Abstract:


Background: Epithelial-mesenchymal transformation (EMT) promotes cancer metastasis including hepatocellular carcinoma. Therefore, EMT-related gene signature was explored.

Objective: The present study was designed to develop an EMT-related gene signature for predicting the prognosis of patients with hepatocellular carcinoma.

Methods: We conducted an integrated gene expression analysis based on tumor data of the patients with hepatocellular carcinoma from The Cancer Genome Atlas (TCGA), HCCDB18 and GSE14520 dataset. An EMT-related gene signature was constructed by least absolute shrinkage and selection operator (LASSO) and COX regression analysis of univariate and multivariate survival.

Results: A 3-EMT gene signature was developed and validated based on gene expression profiles of hepatocellular carcinoma from three microarray platforms. Patients with a high risk score had a significantly worse overall survival (OS) than those with low risk scores. The EMT-related gene signature showed a high performance in accurately predicting prognosis and in examining the clinical characteristics and immune score analysis. Univariate and multivariate Cox regression analyses confirmed that the EMT-related gene signature was an independent prognostic factor for predicting survival in hepatocellular carcinoma patients. Compared with the existing models, our EMT-related gene signature reached higher area under curve (AUC).

Conclusion: Our findings provide novel insight into understanding EMT and help identify hepatocellular carcinoma patients with poor prognosis.

Keywords:

Hepatocellular carcinoma, Epithelial-mesenchymal transformation, signature, prognosis, Bioinformatics

Affiliation:

College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, College of Pharmacy, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065, Key Laboratory for Chinese Medicine Resource and Compound Prescription of Ministry of Education, Hubei University of Chinese Medicine, Wuhan, Hubei, 430065



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