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Fluorescence Assays for High-Throughput Screening of Protein Kinases

[ Vol. 6 , Issue. 4 ]

Author(s):

G. J.R. Zaman, A. Garritsen, Th. de Boer and C. A.A. van Boeckel   Pages 313 - 320 ( 8 )

Abstract:


Protein kinases comprise one of the most important group of targets for drug discovery research today. Methods to identify novel kinase inhibitors by high-throughput screening have evolved rapidly in recent years. An important aspect is the availability of fluorescent probes that can be applied in a homogeneous, or mix-and-measure, assay format. Here, we illustrate the application of fluorescence read-out technologies for kinase targets in light of our own experiences in assay development and high-throughput screening.

Keywords:

fluorescence assays, high-throughput screening, protein kinases, novel kinase inhibitors

Affiliation:

Lead Discovery Unit, N.V. Organon, P.O. Box 20, 5340 BH Oss, The Netherlands.



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