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Applications of luminous bacteria enzymes in toxicology

[ Vol. 18 , Issue. 10 ]

Author(s):

Valentina A. Kratasyuk and Elena N. Esimbekova   Pages 952 - 959 ( 8 )

Abstract:


This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.

Keywords:

Bioluminescence, bioluminescent toxicity enzymatic assay, immobilization of enzymes, luciferase, total toxicity.

Affiliation:

Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, 660036, Russia.



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