Valentina A. Kratasyuk and Elena N. Esimbekova Pages 952 - 959 ( 8 )
This review describes the principle and applications of bioluminescent enzymatic toxicity bioassays. This type of assays uses bacterial coupled enzyme systems: NADH:FMN-oxidoreductase and luciferase to replace living organisms in developing cost-competitive biosensors for environmental, medical and industrial applications. These biosensors instantly signal chemical and biological hazards and allow for detecting a great amount of toxic compounds with advantages associated with fast results, high sensitivity, simplicity, low cost and safety of the procedure.
Bioluminescence, bioluminescent toxicity enzymatic assay, immobilization of enzymes, luciferase, total toxicity.
Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, 660036, Russia.