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Fluorescence Polarization Assays in Signal Transduction Discovery

[ Vol. 6 , Issue. 3 ]

Author(s):

J. Richard Sportsman, Janet Daijo and Elizabeth A. Gaudet   Pages 195 - 200 ( 6 )

Abstract:


Fluorescence polarization (FP) has become widely employed for high throughput screening used in pharmaceutical drug discovery. Assays of important signal transduction targets are now adapted to FP. In this review we examine assays for cyclic adenosine monophosphate, phosphodiesterases, and protein kinases and phosphatases using FP competitive immunoassays and a direct enzymatic method called IMAP.

Keywords:

Fluorescence Polarization, adenosine monophosphate, phosphodiesterases

Affiliation:

Molecular Devices Corporation, Sunnyvale, CA, USA



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