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Fluorescence Polarization Immunoassays for Pesticides

[ Vol. 6 , Issue. 3 ]

Author(s):

Sergei A. Eremin and David S. Smith   Pages 257 - 266 ( 10 )

Abstract:


Fluorescence polarization immunoassay methods for the detection of pesticides and their metabolites or degradation products are reviewed. Advantages and limitations for application to pesticide detection in environmental and food samples are discussed. The influence of the structure of fluorescentlabeled tracers and the affinity and specificity of antibodies on analytical performance is examined. The methods are simple, readily automated, and rapid (total time for assay of a water sample is about 1 min) with sensitivity of 1 - 10 ng / ml pesticide in 0.01 - 0.1 ml sample.

Keywords:

Fluorescence polarization immunoassay,, pesticide,, review.

Affiliation:

Center of Molecular Medicine, Department of Chemical Enzymology, M.V.LomonosovMoscow State University, Leninsky Gory, Moscow, 119899, Russia and MicroPharm Ltd., St. Bartholomew's Hospital, 51-53 Bartholomew Close, London, EC1A 7BE, UK



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